yeast extract 3d graphene nickel foam Search Results


t5 caption a7 yeast strains kanb 3a 3b 3c 3d 3e 4c 4d pos itc flc c albicans atcc 10231  (ATCC)
97
ATCC t5 caption a7 yeast strains kanb 3a 3b 3c 3d 3e 4c 4d pos itc flc c albicans atcc 10231
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
T5 Caption A7 Yeast Strains Kanb 3a 3b 3c 3d 3e 4c 4d Pos Itc Flc C Albicans Atcc 10231, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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yeast laf-3  (Parmalat USA Corp)
90
Parmalat USA Corp yeast laf-3
MIC Values ( μ g/mL) a of KANA, <t> KANB, </t> 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains
Yeast Laf 3, supplied by Parmalat USA Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ivis spectrumct in vivo imaging system  (Revvity)
92
Revvity ivis spectrumct in vivo imaging system
Lung colonization assay with MET −/− A549 cells. Luciferase-expressing wild-type and MET −/− A549 cells were injected into the tail vein of hHGF-KI mice. ( a ) <t>IVIS</t> analysis of mice performed 4 h post-injection (day zero) and then after 4-8-16 days. Each time point represents the mean value of the group. Bars represent SEM. ( b ) IVIS analysis of lungs excised from mice at day 36. Each dot represents the value of the lungs excised from one mouse. Black and red lines: average value for each group. Bars represent SEM. The blue line indicates the threshold (10 4 ) below which IVIS values are considered negative. *, p ≤ 0.05; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.
Ivis Spectrumct In Vivo Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ivis spectrumct in vivo imaging system - by Bioz Stars, 2026-09
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agar ma medium  (Thermo Fisher)
99
Thermo Fisher agar ma medium
Lung colonization assay with MET −/− A549 cells. Luciferase-expressing wild-type and MET −/− A549 cells were injected into the tail vein of hHGF-KI mice. ( a ) <t>IVIS</t> analysis of mice performed 4 h post-injection (day zero) and then after 4-8-16 days. Each time point represents the mean value of the group. Bars represent SEM. ( b ) IVIS analysis of lungs excised from mice at day 36. Each dot represents the value of the lungs excised from one mouse. Black and red lines: average value for each group. Bars represent SEM. The blue line indicates the threshold (10 4 ) below which IVIS values are considered negative. *, p ≤ 0.05; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.
Agar Ma Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3d renderings  (Oxford Instruments)
99
Oxford Instruments 3d renderings
Lung colonization assay with MET −/− A549 cells. Luciferase-expressing wild-type and MET −/− A549 cells were injected into the tail vein of hHGF-KI mice. ( a ) <t>IVIS</t> analysis of mice performed 4 h post-injection (day zero) and then after 4-8-16 days. Each time point represents the mean value of the group. Bars represent SEM. ( b ) IVIS analysis of lungs excised from mice at day 36. Each dot represents the value of the lungs excised from one mouse. Black and red lines: average value for each group. Bars represent SEM. The blue line indicates the threshold (10 4 ) below which IVIS values are considered negative. *, p ≤ 0.05; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.
3d Renderings, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ivis spectrum in vivo imaging system  (Revvity)
96
Revvity ivis spectrum in vivo imaging system
Lung colonization assay with MET −/− A549 cells. Luciferase-expressing wild-type and MET −/− A549 cells were injected into the tail vein of hHGF-KI mice. ( a ) <t>IVIS</t> analysis of mice performed 4 h post-injection (day zero) and then after 4-8-16 days. Each time point represents the mean value of the group. Bars represent SEM. ( b ) IVIS analysis of lungs excised from mice at day 36. Each dot represents the value of the lungs excised from one mouse. Black and red lines: average value for each group. Bars represent SEM. The blue line indicates the threshold (10 4 ) below which IVIS values are considered negative. *, p ≤ 0.05; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.
Ivis Spectrum In Vivo Imaging System, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yeast+extract+3d+graphene+nickel+foam/IVIS+optical+imaging+platform/pmc05892728-18-8-14
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ivis spectrum in vivo imaging system - by Bioz Stars, 2026-09
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ivis spectrum  (Revvity)
94
Revvity ivis spectrum
Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) <t>IVIS</t> image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).
Ivis Spectrum, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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recombinant proteins cb 5083 selleck s8101 bortezomib selleck s1013 n dodecyl b d maltoside amresco j424 diekekrrsenpea  (Selleck Chemicals)
96
Selleck Chemicals recombinant proteins cb 5083 selleck s8101 bortezomib selleck s1013 n dodecyl b d maltoside amresco j424 diekekrrsenpea
Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) <t>IVIS</t> image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).
Recombinant Proteins Cb 5083 Selleck S8101 Bortezomib Selleck S1013 N Dodecyl B D Maltoside Amresco J424 Diekekrrsenpea, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
recombinant proteins cb 5083 selleck s8101 bortezomib selleck s1013 n dodecyl b d maltoside amresco j424 diekekrrsenpea - by Bioz Stars, 2026-09
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vcp inhibitors  (MedChemExpress)
94
MedChemExpress vcp inhibitors
Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) <t>IVIS</t> image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).
Vcp Inhibitors, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yeast+extract+3d+graphene+nickel+foam/VCP+Antibody/bio_rxiv__64898__2026__02__17__706203-81-12-16
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5241 3 d galactose usbiological g1030 yeast nitrogen base qbiogene  (Valiant Co Ltd)
92
Valiant Co Ltd 5241 3 d galactose usbiological g1030 yeast nitrogen base qbiogene
Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) <t>IVIS</t> image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).
5241 3 D Galactose Usbiological G1030 Yeast Nitrogen Base Qbiogene, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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facultative anaerobic growing yeast s cerevisiae ccos 538  (ATCC)
92
ATCC facultative anaerobic growing yeast s cerevisiae ccos 538
Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) <t>IVIS</t> image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).
Facultative Anaerobic Growing Yeast S Cerevisiae Ccos 538, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/yeast+extract+3d+graphene+nickel+foam/Saccharomyces+cerevisiae+Meyen+ex+E%2EC%2E+Hansen/pmc08012708-288-10-18
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isoquercitrin  (ChromaDex)
93
ChromaDex isoquercitrin
Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) <t>IVIS</t> image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).
Isoquercitrin, supplied by ChromaDex, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


MIC Values ( μ g/mL) a of KANA, KANB, 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: MIC Values ( μ g/mL) a of KANA, KANB, 3a–e, and 4c, d against Various Gram-Positive and Gram-Negative Bacterial Strains

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques:

Bar graph displaying the relative initial rates of reactions of the various AMEs with KANB and its derivatives 3a–e and 4c, d. Rates are normalized to KANB.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Bar graph displaying the relative initial rates of reactions of the various AMEs with KANB and its derivatives 3a–e and 4c, d. Rates are normalized to KANB.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques:

MIC Values ( μ g/mL) Determined for KANB, Its Derivatives 3a–e and 4c, d, and Three Control Antifungal Agents (POS, ITC, and FLC) against Various Yeast Strains and Filamentous Fungi a

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: MIC Values ( μ g/mL) Determined for KANB, Its Derivatives 3a–e and 4c, d, and Three Control Antifungal Agents (POS, ITC, and FLC) against Various Yeast Strains and Filamentous Fungi a

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Control

Representative time-kill studies of KANB derivatives 3c and 3d against azole-resistant C. albicans ATCC 64124 (strain B). (A) Cultures were exposed to 3c at 8 μg/mL (○), 16 μg/mL (▼), and 32 μg/mL (△). (B) Cultures were exposed to 3d at 2 μg/mL (○), 4 μg/mL (▼), and 8 μg/mL (△). In both panels, cultures were exposed to AmB at 1 μg/mL (■) or to a no drug control (●).

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Representative time-kill studies of KANB derivatives 3c and 3d against azole-resistant C. albicans ATCC 64124 (strain B). (A) Cultures were exposed to 3c at 8 μg/mL (○), 16 μg/mL (▼), and 32 μg/mL (△). (B) Cultures were exposed to 3d at 2 μg/mL (○), 4 μg/mL (▼), and 8 μg/mL (△). In both panels, cultures were exposed to AmB at 1 μg/mL (■) or to a no drug control (●).

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Control

(A) Representative dose-dependent membrane permeabilization effects of KANB and its derivatives 3c and 3d on azole-resistant C. albicans ATCC 64124 (B). From top to bottom: Propidium iodine (PI) dye uptake by yeast cells without drug, with KANB (62.5 μg/mL), with 3c (1× and 2× MIC), and with 3d (1× and 2× MIC). (B) Quantitative representation of the images shown in panel A.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: (A) Representative dose-dependent membrane permeabilization effects of KANB and its derivatives 3c and 3d on azole-resistant C. albicans ATCC 64124 (B). From top to bottom: Propidium iodine (PI) dye uptake by yeast cells without drug, with KANB (62.5 μg/mL), with 3c (1× and 2× MIC), and with 3d (1× and 2× MIC). (B) Quantitative representation of the images shown in panel A.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Membrane

Hemolytic activity of KANB, gramicidin, amphotericin B (AmB), and 3a–e on mouse red blood cells.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Hemolytic activity of KANB, gramicidin, amphotericin B (AmB), and 3a–e on mouse red blood cells.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques: Activity Assay

Mammalian cell cytotoxicity of KANB and its derivatives 3a–d against (A) A549 cell line and (B) BEAS-2B cell line.

Journal: Journal of medicinal chemistry

Article Title: Synthesis and Bioactivities of Kanamycin B-Derived Cationic Amphiphiles

doi: 10.1021/acs.jmedchem.5b01375

Figure Lengend Snippet: Mammalian cell cytotoxicity of KANB and its derivatives 3a–d against (A) A549 cell line and (B) BEAS-2B cell line.

Article Snippet: This is in agreement with our results showing that, as with bacteria, 3d may also be able to delay the development of resistance by fungi ( Figure S27 ). table ft1 table-wrap mode="anchored" t5 caption a7 yeast strains KANB 3a 3b 3c 3d 3e 4c 4d POS ITC FLC C. albicans ATCC 10231 ( A ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 0.5 0.5 62.5 C. albicans ATCC 64124 ( B ) b >125 >125 125 31.2 7.8 3.9 31.2 3.9 >62.5 >62.5 >125 C. albicans ATCC MYA-2876 ( C ) c >125 >125 125 31.2 7.8 3.9 31.2 3.9 7.8 7.8 15.6 C. albicans ATCC 90819 ( D ) b >125 >125 125 31.2 15.6 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-2310 ( E ) c >125 >125 62.5 7.8 7.8 3.9 62.5 7.8 31.2 31.2 >125 C. albicans ATCC MYA-1237 ( F ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 C. albicans ATCC MYA-1003 ( G ) b >125 >125 125 31.2 7.8 3.9 62.5 7.8 15.6 31.2 62.5 Filamentous Fungi Aspergillus nidulans ATCC 38163 ( H ) >125 15.6 ≤1.95 ≤1.95 ≤1.95 1.95 3.9 1.95 ≤1.95 ≤1.95 >62.5 Open in a separate window a All experiments were performed in duplicate.

Techniques:

Lung colonization assay with MET −/− A549 cells. Luciferase-expressing wild-type and MET −/− A549 cells were injected into the tail vein of hHGF-KI mice. ( a ) IVIS analysis of mice performed 4 h post-injection (day zero) and then after 4-8-16 days. Each time point represents the mean value of the group. Bars represent SEM. ( b ) IVIS analysis of lungs excised from mice at day 36. Each dot represents the value of the lungs excised from one mouse. Black and red lines: average value for each group. Bars represent SEM. The blue line indicates the threshold (10 4 ) below which IVIS values are considered negative. *, p ≤ 0.05; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.

Journal: Cancers

Article Title: Genetic Ablation of the MET Oncogene Defines a Crucial Role of the HGF/MET Axis in Cell-Autonomous Functions Driving Tumor Dissemination

doi: 10.3390/cancers15102742

Figure Lengend Snippet: Lung colonization assay with MET −/− A549 cells. Luciferase-expressing wild-type and MET −/− A549 cells were injected into the tail vein of hHGF-KI mice. ( a ) IVIS analysis of mice performed 4 h post-injection (day zero) and then after 4-8-16 days. Each time point represents the mean value of the group. Bars represent SEM. ( b ) IVIS analysis of lungs excised from mice at day 36. Each dot represents the value of the lungs excised from one mouse. Black and red lines: average value for each group. Bars represent SEM. The blue line indicates the threshold (10 4 ) below which IVIS values are considered negative. *, p ≤ 0.05; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.

Article Snippet: XenoLight D-Luciferin (150 mg/kg) was injected intraperitoneally in mice 4 h, 4, 8, and 16 days after cells injection, and the bioluminescent signal was measured by IVIS SpectrumCT in vivo imaging system (PerkinElmer Inc.) on live animals.

Techniques: Luciferase, Expressing, Injection

In vivo analysis of MET −/− Capan-I tumors and metastasis. Luciferase-expressing wild-type and MET −/− Capan-I cells were injected into the pancreas of hHGF-KI mice. ( a ) IVIS analysis of mice performed 3, 21, and 35 days post-injection. Each time point represents the mean value of the group. Bars represent SEM. ( b – d ) IVIS analysis of isolated organs (pancreas, livers, and lungs) excised from mice at day 35. Each dot represents the value of the organ excised from one mouse. The blue lines indicate the threshold (10 4 ) below which IVIS values are considered negative. ****, p ≤ 0.0001; ***, p ≤ 0.001; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.

Journal: Cancers

Article Title: Genetic Ablation of the MET Oncogene Defines a Crucial Role of the HGF/MET Axis in Cell-Autonomous Functions Driving Tumor Dissemination

doi: 10.3390/cancers15102742

Figure Lengend Snippet: In vivo analysis of MET −/− Capan-I tumors and metastasis. Luciferase-expressing wild-type and MET −/− Capan-I cells were injected into the pancreas of hHGF-KI mice. ( a ) IVIS analysis of mice performed 3, 21, and 35 days post-injection. Each time point represents the mean value of the group. Bars represent SEM. ( b – d ) IVIS analysis of isolated organs (pancreas, livers, and lungs) excised from mice at day 35. Each dot represents the value of the organ excised from one mouse. The blue lines indicate the threshold (10 4 ) below which IVIS values are considered negative. ****, p ≤ 0.0001; ***, p ≤ 0.001; **, p ≤ 0.01. The data reported in the figure are representative of two experiments.

Article Snippet: XenoLight D-Luciferin (150 mg/kg) was injected intraperitoneally in mice 4 h, 4, 8, and 16 days after cells injection, and the bioluminescent signal was measured by IVIS SpectrumCT in vivo imaging system (PerkinElmer Inc.) on live animals.

Techniques: In Vivo, Luciferase, Expressing, Injection, Isolation

Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) IVIS image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).

Journal: Advanced Science

Article Title: An IL‐12‐Based Nanocytokine Safely Potentiates Anticancer Immunity through Spatiotemporal Control of Inflammation to Eradicate Advanced Cold Tumors

doi: 10.1002/advs.202205139

Figure Lengend Snippet: Nano‐IL‐12 improves pharmacokinetics and anti‐tumor efficacy. a) IVCLSM images of the earlobe skin of mice after i.v. injection of 10 µg A647‐labeled IL‐12 or Nano‐IL‐12 (red color). Scale bar = 50 µm. Mean fluorescence intensity in the tissue area (white boxes) at 5 h after injection were quantified and normalized to the maximum intensity in the vasculature immediately after injection ( V max ). b) Blood circulation profiles of free IL‐12 and Nano‐IL‐12 after i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 determined by ELISA. Also, the concentration of released IL‐12 from Nano‐IL‐12 in blood is plotted (data are shown as mean ± S.D., n = 5 mice per group). c) IVIS image of B16F10 melanoma tumors excised 24 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 labeled with A647. d) Quantification of the IL‐12 level in 4T1 TNBC tumors at 24‐ and 48 h post i.v. injection of 10 µg IL‐12 or equivalent Nano‐IL‐12 by ELISA (Data are shown as mean ± S.D.; n = 3 mice per group; p values are calculated by one‐way ANOVA). e) Anti‐tumor activity of a single i.v. injection (injection days are indicated by the arrow (Day 8 for B16F10 model and Day 7 for 4T1 model)) of 10 µg IL‐12 or equivalent Nano‐IL‐12. The results in B16F10 melanoma are shown in the upper panel and the results in the 4T1 TNBC are shown in the lower panel. The individual tumor growth curves are shown in the left panels. The average tumor volumes curves are shown in the center panels, and the survival curves are shown in the right panel (Data are shown as mean ± SEM; n = 5 mice per group, p values are calculated via log‐rank analysis).

Article Snippet: The earlobe skin of the mice was observed by in vivo confocal laser scanning microscopy (IVCLSM) (A1R confocal LSM, Nikon, Japan) continuously for 5 h, and the melanoma tumors were excised after 24 h for fluorescent imaging by in vivo imaging system (IVIS Spectrum, PerkinElmer, USA).

Techniques: Drug discovery, Injection, Labeling, Fluorescence, Enzyme-linked Immunosorbent Assay, Concentration Assay, Activity Assay